Journal: Oncotarget

Article Title: Proteomic Signatures of the Desmoplastic Invasion Front Reveal Collagen Type XII as a Marker of Myofibroblastic Differentiation During Colorectal Cancer Metastasis

doi:

Figure Lengend Snippet: (A-C) Collagen III immunohistochemical expression in the stromal collagen and myofibroblasts (arrows) around the tumor invasion front in cases of CRC (D-F) Collagen XII immunohistochemical expression in the stromal collagen and myofibroblasts (arrows) around the tumor invasion front in the same cases of CRC, respectively, and in tumor cells of the tumor invasion front (arrowheads). Magnifications: A, D, F x200 and B, C, E x400 .

Article Snippet: The following antibodies were purchased: mouse monoclonal anti-CK (cytokeratin cocktail) from Cell Marque (clone A1E&AE3), mouse monoclonal anti-alpha-SMA from DAKO (clone 1A4), mouse monoclonal anti-collagen-III from Biogenex (clone HWD1.1; concentrated in PBS), mouse monoclonal anti-collagen-XII from Sigma-Aldrich (clone 1303).

Techniques: Immunohistochemical staining, Expressing

Journal: Oncotarget

Article Title: Proteomic Signatures of the Desmoplastic Invasion Front Reveal Collagen Type XII as a Marker of Myofibroblastic Differentiation During Colorectal Cancer Metastasis

doi:

Figure Lengend Snippet: (A) Cells lining the invasion front (encircled) and tumor budding cells (arrows) were found positive for collagen type XII and negative for collagen type III. (B) Mean ratios of Collagens type III and XII, calculated from gene expression meta-analysis between colorectal cancer adenocarcinomas and normal colonic mucosa in a total of 6 experiments in the Genevestigator database. (C) Mean COL12A1 expression in cancerous lesions of 24 CRC patients and adjacent to the tumor normal colonic mucosa. All bars are mean values with standard deviations calculated from all experiments or replicates .

Article Snippet: The following antibodies were purchased: mouse monoclonal anti-CK (cytokeratin cocktail) from Cell Marque (clone A1E&AE3), mouse monoclonal anti-alpha-SMA from DAKO (clone 1A4), mouse monoclonal anti-collagen-III from Biogenex (clone HWD1.1; concentrated in PBS), mouse monoclonal anti-collagen-XII from Sigma-Aldrich (clone 1303).

Techniques: Expressing

Journal: Biomedicines

Article Title: Therapeutic Effects of Omentin-1 on Pulmonary Fibrosis by Attenuating Fibroblast Activation via AMP-Activated Protein Kinase Pathway

doi: 10.3390/biomedicines10112715

Figure Lengend Snippet: The effect of omentin-1 deficiency on BLM-induced lung fibrosis at 21 d. ( A ) Experimental design: BLM (1.0 mg/kg) was injected to induce fibrosis (21 days) in wild-type and Omentin-1 −/− mice. ( B ) Weight change of mice 21 days after BLM challenge ( n = 10). ( C ) Representative micrographs of H&E staining of lung tissue (Bar = 50 μm, n = 5). ( D ) Representative micrographs of Masson trichrome staining, immunohistochemical staining of lung sections for Collagen III (Col3) and α-SMA (Bar = 50 μm, n = 5). (E) Ashcroft score to grade fibrosis scale ( n = 5). ( F ) Collagen content was measured by hydroxyproline (HYP) assay ( n = 5). ( G ) The content of collagen III (Col3) and α-SMA in lung tissue detected by Western blotting with cropping gel at the relative molecular size. ( n = 3). ( H ) Semi-quantitative analysis of Col3 level evaluated by Western blotting. ( I ) Semi-quantitative analysis of α-SMA level evaluated by Western blotting. The data are presented as the mean ± SD. * p < 0.05, ** p < 0.01 vs. control group. # p < 0.05, ## p < 0.01 vs. BLM group.

Article Snippet: The cells were blocked with 5% BSA for 30 min, and stained overnight at 4 °C with the primary antibodies, i.e., mouse anti-α-SMA (1:100; Servicebio, Wuhan, China) and mouse anti- collagen III (1:100; Servicebio, Wuhan, China), followed by staining with Alexa Fluor 594-goat anti-mouse secondary antibodies (1:100, Beyotime, Shanghai, China) at room temperature for 30 min.

Techniques: Injection, Staining, Immunohistochemical staining, Western Blot, Control